Apart from acting in the plays written by acclaimed playwrights such as Vijay Tendulkar, Deshpande featured in several Marathi and Hindi films and TV serials.
In Hindi, the noteworthy films in which she appeared included ‘Bhumika’ (1977), ‘Arvind Desai Ki Ajeeb Dastaan’ (1978) and ‘Gaman’ (1978). In recent years, she had appeared in Gauri Shinde-directed ‘English-Vinglish’.
She was associated with ‘Rangayan’, a pioneering Marathi experimental theatre group, alongwith Tendulkar, Vijaya Mehta, and Satyadev Dubey. In 1971 she co-founded the theatre group `Awishkar’ with her husband Arvind Deshpande (who died in 1987).