Amanda Perreault appeared in court on Tuesday on charges of sexual assault, sexual interference, luring to commit invitation to sexual touching and making sexually explicit material available to a child.
The Dufferin-Peel Catholic District School Board said in a statement the accused has been suspended with pay pending the resolution of the charges against her.
Perreault was hired as an occasional teacher at the board in 2009 and was a Grade 2 and 3 Long-Term Occasional Teacher at St. James since the 2014-15 school year.
School board officials say she also had assignments at three other schools in the past four years which included St. Lucy and Bishop Francis Allan in Brampton and Sts. Martha and Mary in Mississauga.
Police say they are concerned that there may be more victims and are asking anyone with information to come forward.