The vehicle clipped the mirror of a northbound Hyundai SUV before hitting a delivery truck head on.
The driver of the Lexus was pronounced dead at the scene. The other drivers were not injured.
Roads in the area – including the highway exit to St. Jacobs as well as the village’s exit on the roundabout – were closed for several hours while police investigated and a fuel spill was cleaned up. Some reports online indicated traffic on the highway was backed up to University Avenue in Waterloo.
The woman’s name was not released.
Officers continue to investigate the cause of the collision and ask anyone with information to call Waterloo Regional Police at 519-653-7700.