As the biker was passing Vienna Line it collided with a westbound Chrysler van.
The driver of the motorcycle, a 41-year-old man from Bayham Township, died at the scene. His identity is being withheld pending notification of next-of-kin.
The passenger on the motorcycle was transported to hospital with life-threatening injuries.
The four occupants of the van were transported to hospital for non-life threatening injuries.
Police say westbound traffic on Vienna Line is governed by a stop sign. The crash remains under investigation.