The 19-year-old driver of the passenger vehicle was pronounced dead at the scene.
Police said the driver and the passenger travelling in the tractor trailer, both 53-year-old men, were taken to hospital with non-life threatening injuries.
Highway 17 is expected to remain closed between Black Bay Road and Paquette Road until late Saturday afternoon while police investigate.
Detours are in place.
Police say the name of the victim is not being released until family have been notified.