The rider died at the scene and is yet to be formally identified.
The driver of the ute, a 57-year-old Wilby man, did not suffer any injuries and is assisting police.
His death brings the state road toll to 135, up from 117 this time last year.
Meanwhile, an 18-year-old Hurstbridge man remains in a critical condition at the Alfred after a crash on an isolated dirt road in Melbourne’s east.
Emergency services were called to the crash shortly after 7.30pm on Sunday by a passer-by who noticed the wreckage of a car on a popular 4×4 track in Toolangi, 90 minutes east of Melbourne.
The car’s 46-year-old driver died at the scene after apparently losing control of the vehicle, rolling it and crashing into a tree beside Sylvia Creek road.
The 18-year-old passenger was flown to hospital in Melbourne.
It is not yet clear how long the two men, who are both from Hurstbridge, had been stuck in the car before help arrived.
Police will prepare a report for the Coroner.