One report said both were doctors who did work at a Kabul hospital not far from where they were taken on Sunday night, Kabul time.
A spokesman from the Department of Foreign Affairs told AAP the Australian embassy in Afghanistan was seeking to verify reports of the kidnapping.
The US State Department is aware of the reports.
“We are aware of reports of a US citizen kidnapped in Kabul. Due to privacy considerations, we have no information to offer,” the State Department said in a media statement.
ABC America said Kabul’s police chief Abdel Rahman Rahimi had confirmed the abductions.
The university is a private, not-for-profit university, which has received millions from the US Agency for International Development.